8B5W
Crystal structure of the E3 module from UBR4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2820 or 1.2831 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.131, 84.644, 148.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.070 - 1.800 |
| R-factor | 0.1993 |
| Rwork | 0.198 |
| R-free | 0.22160 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.543 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | CRANK2 (2.01) |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.070 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.024 | 0.291 |
| Rmeas | 0.034 | 0.411 |
| Rpim | 0.024 | 0.291 |
| Number of reflections | 36081 | 3577 |
| <I/σ(I)> | 23.84 | 2.56 |
| Completeness [%] | 91.3 | 91.67 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10 mM Na2HPO4 13% PEG20000 |
