8AXI
Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.920, 51.510, 118.470 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 50.210 - 1.250 |
| R-factor | 0.1616 |
| Rwork | 0.161 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.510 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.050 | 0.647 |
| Rmeas | 0.054 | 0.713 |
| Number of reflections | 323513 | 17780 |
| <I/σ(I)> | 16.27 | 2.51 |
| Completeness [%] | 93.6 | 69.8 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | MgCl2, BIS-TRIS pH 5.5, Polyethylene glycol 3350 |
