8AW1
c-MET Y1235D mutant in complex with Tepotinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.833, 43.291, 89.286 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 44.640 - 2.140 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r1v |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.8 (8-JUN-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.280 | 2.390 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmeas | 0.068 | 0.540 |
| Number of reflections | 32217 | 9089 |
| <I/σ(I)> | 12.91 | |
| Completeness [%] | 96.6 | 97.6 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | PEG 8,000 |
