8AS9

Crystal structure of the talin-KANK1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	H 3 2
Unit cell lengths	207.022, 207.022, 151.859
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.760 - 3.400
R-factor	0.2472
Rwork	0.245
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xyx
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.890	3.580
High resolution limit [Å]	3.400	3.400
Rpim		1.356
Number of reflections	17332	2505
<I/σ(I)>	2.9	0.5
Completeness [%]	100.0	100
Redundancy	20.9	21.8
CC(1/2)	0.900	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	1M Ammonium sulfate, 0.1M CHES 9.5, 0.2M Sodium chloride, 6 % (v/v) Glycerol