8ARP

Crystal structure of DEAD-box protein Dbp2 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979300
Spacegroup name	P 21 21 21
Unit cell lengths	145.939, 168.843, 168.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.400 - 3.050
R-factor	0.6542
Rwork	0.223
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ark
RMSD bond length	0.005
RMSD bond angle	0.998
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.400	3.099
High resolution limit [Å]	3.046	3.046
Rmerge	0.153	1.284
Rmeas	0.159	1.355
Rpim	0.044	0.362
Number of reflections	1019758	3975
<I/σ(I)>	11.2	2.1
Completeness [%]	96.2	100
Redundancy	13.2	13.4
CC(1/2)	0.998	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	2.02 M (NH4)2SO4 100 mM Tris pH 7.0 150 mM Li2SO4