8AHY
X-ray structure of human NCS-1 bound to Ric-8A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979263 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.635, 56.635, 135.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.240 - 1.700 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.20760 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.774 |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.240 | 1.784 |
High resolution limit [Å] | 1.700 | 1.704 |
Number of reflections | 44184 | 1101 |
<I/σ(I)> | 13.6 | |
Completeness [%] | 96.0 | 66.4 |
Redundancy | 25.5 | |
CC(1/2) | 0.997 | 0.538 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 30 % PEG 4000, 100 mM sodium acetate pH 4.6, 100 mM magnesium chloride |