8AG1
Crystal structure of a novel OX40 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-01 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 162.356, 162.356, 232.145 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.502 - 3.303 |
| Rwork | 0.194 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2EHY |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.593 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (8.0.002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.502 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.168 | 1.945 |
| Number of reflections | 27927 | 4370 |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 99.7 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.994 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 293 | 0.1m CITRATE, PH5.6, 2% TACSIMATE, 16% PEG3350 |
