8AEZ

X-ray structure of the deglycosylated receptor binding domain of Env glycoprotein of Simian Foamy virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 32 2 1
Unit cell lengths	99.451, 99.451, 120.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.730 - 2.574
R-factor	0.2153
Rwork	0.213
R-free	0.25300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS (`)
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.730	2.666
High resolution limit [Å]	2.574	2.574
Rmerge	0.205	0.936
Rpim	0.047	0.212
Number of reflections	22363	2101
<I/σ(I)>	10.1	1.85
Completeness [%]	99.9
Redundancy	20.3
CC(1/2)	0.986	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.1M Tris pH 8.5, 3.5M sodium formate