8A3V

Crystal structure of the Vibrio cholerae replicative helicase (VcDnaB) in complex with its loader protein (VcDciA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.984
Spacegroup name	H 3 2
Unit cell lengths	186.515, 186.515, 252.839
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.800 - 2.900
R-factor	0.2798
Rwork	0.279
R-free	0.29010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t66
RMSD bond length	0.005
RMSD bond angle	0.720
Data reduction software	XDS (VERSION Mar 17, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (Vers 11.7.03; 13.07.2020)
Refinement software	BUSTER (2.10.3 (20-MAY-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.260	48.260	3.120
High resolution limit [Å]	2.900	9.970	2.900
Rmerge	0.196	0.035	2.360
Rmeas	0.201	0.036	2.421
Rpim	0.046	0.009	0.541
Number of reflections	21186	1000	1000
<I/σ(I)>	13.4	61.3	1.6
Completeness [%]	94.1	100	71.7
Redundancy	17.4	16.3	19.9
CC(1/2)	0.999	1.000	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	291	0.1M Sodium Acetate 0.9M Potassium/Sodium Tartrate