8A29
Apo 1-deoxy-D-xylulose 5-phosphate synthase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.444, 137.631, 232.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.590 - 2.100 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ouv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.590 | 2.279 |
| High resolution limit [Å] | 2.100 | 2.200 |
| Rmerge | 0.058 | 0.294 |
| Number of reflections | 216635 | 18663 |
| <I/σ(I)> | 11.09 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES, 12% PEG 8000, 200 mM calcium acetate |
