8A12
Plasmodium falciparum Myosin A full-length, post-rigor state complexed to Mg.ATP-gamma-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-02-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.125, 115.140, 170.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.560 - 2.030 |
R-factor | 0.1882 |
Rwork | 0.186 |
R-free | 0.23450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ycy |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.112 | 2.223 |
High resolution limit [Å] | 2.027 | 2.027 |
Rmerge | 0.078 | 1.136 |
Number of reflections | 87835 | 4371 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 94.4 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277.4 | 1,6 M Ammonium sulfate; 0,1 M HEPES pH 7,5; 4% PEG400 |