7ZX2

Tubulin-Pelophen B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	104.709, 156.973, 181.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.660 - 2.500
R-factor	0.199
Rwork	0.197
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o2b
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.660	49.660	2.540
High resolution limit [Å]	2.500	13.690	2.500
Rmerge	0.098	0.071	1.038
Rmeas	0.105	0.077	1.108
Rpim	0.037	0.030	0.384
Total number of observations	841601	4842	42221
Number of reflections	103744	728	5073
<I/σ(I)>	13.5	34	2
Completeness [%]	100.0	98.2	100
Redundancy	8.1	6.7	8.3
CC(1/2)	0.998	0.994	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Mes, imidazole, calcium chloride, magnesium chloride, L-tyrosine, glycerol, PEG 4000