7ZWN
Crystal structure of human BCL6 BTB domain in complex with a WVIP peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-27 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.5419 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 66.453, 66.453, 151.203 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.110 - 2.050 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bim |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.790 | 45.790 | 2.110 |
High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
Rmerge | 0.175 | 0.046 | 1.243 |
Rmeas | 0.180 | 0.048 | 1.305 |
Rpim | 0.040 | 0.010 | 0.390 |
Total number of observations | 254697 | 3915 | 10475 |
Number of reflections | 13158 | 219 | 986 |
<I/σ(I)> | 12.8 | 34 | 1.9 |
Completeness [%] | 100.0 | 99.1 | 99.9 |
Redundancy | 19.4 | 17.9 | 10.6 |
CC(1/2) | 0.998 | 0.999 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2 microliter of the BCL6-BTB/WVIP complex at 4 mg/mL plus 1 microliter of a crystallisation solution consisting of 1 M K2HPO4, 0.7 M NaH2PO4, 75 mM sodium acetate buffer pH 4.5 and 2 % DMSO, against 350 microliter of crystallisation solution. |