7ZWI
Pfs48/45 C-terminal domain bound to fab fragment of monoclonal antibody 32F3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.659, 87.104, 110.620 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 77.570 - 1.900 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h5n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-APR-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.570 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.037 | 0.282 |
| Number of reflections | 781771 | 36476 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04M potassium dihydrogen phosphate, 16% (w/v) polyethylene glycol (PEG) 8000 and 20 % (v/v) glycerol |
