7ZWF

Pfs48/45 bound to scFv fragment of monoclonal antibody 32F3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97949
Spacegroup name	C 2 2 21
Unit cell lengths	81.826, 126.115, 146.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.910 - 2.132
R-factor	0.2762
Rwork	0.276
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h5n
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (20-OCT-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.390	2.170
High resolution limit [Å]	2.130	2.130
Rpim	0.037	2.260
Number of reflections	586552	28745
<I/σ(I)>	11.2
Completeness [%]	99.8
Redundancy	13.8
CC(1/2)	0.999	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M MES pH6.0, 0.2M CaCl2, 20% PEG6000