7ZVB
Crystal Structure of the mature form of the glutamic-class prolyl-endopeptidase neprosin at 2.35 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0050 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.760, 49.950, 59.860 |
Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
Resolution | 52.490 - 2.350 |
R-factor | 0.1972 |
Rwork | 0.195 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7zu8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.500 | 2.434 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 13077 | 1292 |
<I/σ(I)> | 5.6 | |
Completeness [%] | 99.6 | 98.85 |
Redundancy | 6.46 | |
CC(1/2) | 0.990 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 1000, 10% PEG 8000 |