7ZTL

Crystal structure of a covalently linked Aurora-A N-Myc complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 32 2 1
Unit cell lengths	86.614, 86.614, 91.789
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.170 - 1.900
R-factor	0.1649
Rwork	0.163
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g1x
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.170	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.155	2.516
Rpim	0.035	0.573
Number of reflections	31825	3148
<I/σ(I)>	11.06	1.28
Completeness [%]	99.9
Redundancy	19.8
CC(1/2)	0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1 M TRIS pH 8.5, 0.4 M MgCl2, 25% PEG 3350