7ZTL
Crystal structure of a covalently linked Aurora-A N-Myc complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 86.614, 86.614, 91.789 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.170 - 1.900 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.19310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g1x |
RMSD bond length | 0.008 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.170 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.155 | 2.516 |
Rpim | 0.035 | 0.573 |
Number of reflections | 31825 | 3148 |
<I/σ(I)> | 11.06 | 1.28 |
Completeness [%] | 99.9 | |
Redundancy | 19.8 | |
CC(1/2) | 0.998 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1 M TRIS pH 8.5, 0.4 M MgCl2, 25% PEG 3350 |