7ZRP
2.65 Angstrom crystal structure of Ca/CaM:CaMKIIdelta peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-11 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.283, 72.590, 78.156 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.244 - 2.650 |
Rwork | 0.207 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wel |
RMSD bond length | 0.009 |
RMSD bond angle | 1.288 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.250 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.154 | 1.921 |
Rmeas | 0.185 | 2.300 |
Rpim | 0.102 | 1.249 |
Number of reflections | 11133 | 1442 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 100.0 | |
Redundancy | 5.8 | 6.1 |
CC(1/2) | 0.996 | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 200 mM Zinc acetate, 100 mM Imidazole pH8.0, 18% PEG3000 |