7ZOR
Crystal structure of anti-Siglec-15 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.020, 60.681, 131.258 |
| Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
| Resolution | 62.467 - 3.933 |
| R-factor | 0.2319 |
| Rwork | 0.229 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vkk |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.467 | 4.001 |
| High resolution limit [Å] | 3.933 | 3.933 |
| Rmerge | 0.465 | 0.931 |
| Rmeas | 0.511 | 1.090 |
| Rpim | 0.208 | 0.552 |
| Number of reflections | 8960 | 429 |
| <I/σ(I)> | 6.4 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 | 4.4 |
| CC(1/2) | 0.865 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% (w/v) PEG3350 0.1M HEPES pH7.5 0.2M Magnesium chloride |
