7ZMS

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G4-043

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	81.909, 90.121, 216.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.988 - 2.700
Rwork	0.235
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lb5
RMSD bond length	0.006
RMSD bond angle	1.555
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.210
High resolution limit [Å]	2.170	2.170
Number of reflections	42737	1944
<I/σ(I)>	7.2
Completeness [%]	99.6
Redundancy	13.3
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M sodium chloride -- 25% PEG3350 -- 0.1M HEPES pH 7.5