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7ZMR

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G2*-011

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04-1
Synchrotron siteDiamond
BeamlineI04-1
Temperature [K]100
Detector technologyPIXEL
Collection date2020-01-17
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.9119
Spacegroup nameC 2 2 21
Unit cell lengths114.890, 198.954, 172.926
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution99.493 - 3.300
Rwork0.243
R-free0.29200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5lb5
RMSD bond length0.006
RMSD bond angle1.589
Data reduction softwarexia2
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]99.5403.090
High resolution limit [Å]3.0403.040
Number of reflections385641907
<I/σ(I)>3.4
Completeness [%]100.0
Redundancy6.6
CC(1/2)0.9000.200
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52930.2M lithium sulfate -- 25% PEG3350 -- 0.1M HEPES pH 7.5

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