7ZKC
Catalytic domain of UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2017-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97956 |
Spacegroup name | H 3 |
Unit cell lengths | 118.825, 118.825, 68.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.130 - 1.769 |
R-factor | 0.2061 |
Rwork | 0.205 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nv4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | XDS (JUN 1, 2017) |
Data scaling software | autoPROC (1.0.5) |
Phasing software | MOLREP (11.5.04) |
Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.170 | 1.340 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.080 | 1.397 |
Rmeas | 0.150 | 2.250 |
Number of reflections | 35287 | 5115 |
<I/σ(I)> | 10.5 | 1.1 |
Completeness [%] | 99.0 | 97.7 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.06M Divalents, 0.1 M Buffer System 1, 30% v/v Precipitant Mix 1 (Morpheus Screen condition 1-1) |