7ZJU
Structure of human USPL1 in covalent complex with SUMO3-2Br probe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-22 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.060, 84.833, 71.106 |
Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
Resolution | 54.470 - 2.170 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23780 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.640 |
Data reduction software | xia2 (0.5.902) |
Data scaling software | Aimless (1.12.2) |
Phasing software | CRANK2 (2.0.251) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.470 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.112 | 0.640 |
Rmeas | 0.123 | 0.711 |
Number of reflections | 80896 | 3592 |
<I/σ(I)> | 8.5 | 2.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 5.7 | 5 |
CC(1/2) | 0.996 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 100 mM Tris pH 8.8, 19% PEG6000, 200 mM CaCl2 |