7ZJM

Crystal structure of a complex between CspZ from Borrelia burgdorferi strain B408 and human FH SCR domains 6-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97988
Spacegroup name	P 21 21 21
Unit cell lengths	42.098, 71.069, 147.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.870 - 2.590
R-factor	0.2209
Rwork	0.218
R-free	0.27580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ayd 4cbe
RMSD bond length	0.010
RMSD bond angle	1.548
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.870	2.710
High resolution limit [Å]	2.590	2.590
Rmerge	0.112	0.380
Number of reflections	14404	1708
<I/σ(I)>	12.8	4.9
Completeness [%]	99.5
Redundancy	7.1
CC(1/2)	0.988	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	0.2 M Zinc acetate 0.1 M imidazole (pH 7.4) 10% PEG 3000