7ZIQ

BK Polyomavirus VP1 in complex with 6'-Sialyllactose glycomacromolecules (aromatic linker)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-02-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99
Spacegroup name	P 21 21 2
Unit cell lengths	144.327, 151.838, 63.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 1.900
Rwork	0.187
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mj1
RMSD bond length	0.008
RMSD bond angle	1.492
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.010
High resolution limit [Å]	1.900	1.900
Rmeas	0.190	1.940
Number of reflections	110045	17476
<I/σ(I)>	12.84
Completeness [%]	99.9
Redundancy	13.42
CC(1/2)	0.990	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 0.25 M LiCl, 18% (w/v) PEG 3350