7ZIP
JC Polyomavirus VP1 in complex with 3'-Sialyllactose glycomacromolecules (aliphatic linker)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-02-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.734, 152.505, 62.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.963 - 1.900 |
| Rwork | 0.172 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.130 | 1.270 |
| Number of reflections | 110486 | 17558 |
| <I/σ(I)> | 13.04 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.42 | |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 0.25 M LiCl, 18% (w/v) PEG 3350 |
