7ZHD
Crystal structure of CtaZ in complex with Closthioamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.640, 40.850, 44.810 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.033 | 0.548 |
| Number of reflections | 22520 | 3648 |
| <I/σ(I)> | 15.9 | 2.2 |
| Completeness [%] | 97.9 | 99.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Na-cacodylate, 200 mM ZnAc2, 18% PEG 8000 |
