7ZHB
Catalytic domain of UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum in complex with UDP-glucose (conformation 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978570 |
Spacegroup name | H 3 |
Unit cell lengths | 121.442, 121.442, 62.117 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.260 - 1.890 |
R-factor | 0.1853 |
Rwork | 0.184 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fsn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.3) |
Phasing software | autoBUSTER (2.10.4) |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.720 | 2.030 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.070 | 1.110 |
Rmeas | 0.070 | 1.250 |
Number of reflections | 24990 | 3379 |
<I/σ(I)> | 11 | 1.2 |
Completeness [%] | 96.7 | |
Redundancy | 5 | |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Morpheus condition 1-40 (0.12 M Alcohols, 0.1 M Buffer System 1 pH 482 6.5, 37.5% v/v Precipitant Mix 4) |