7ZH9

Uba1 in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	107.720, 118.064, 196.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.238 - 1.720
R-factor	0.1669
Rwork	0.165
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmm
RMSD bond length	0.010
RMSD bond angle	0.924
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.238	1.705
High resolution limit [Å]	1.705	1.705
Number of reflections	91872	4617
<I/σ(I)>	10.6
Completeness [%]	99.9
Redundancy	6.65
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M ammonium sulfate, 0.1 M HEPES pH 7.5 and 25% PEG 3350