7ZG4

Crystal structure of YTHDF2 with compound YLI_DF_042

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	80.380, 80.380, 114.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.170 - 2.010
R-factor	0.2253
Rwork	0.223
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdn
RMSD bond length	0.007
RMSD bond angle	0.793
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.170	2.130
High resolution limit [Å]	2.010	2.010
Number of reflections	54970	8790
<I/σ(I)>	18.35
Completeness [%]	99.3
Redundancy	4.7
CC(1/2)	0.999	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.1