7ZEE
Human cytosolic 5' nucleotidase IIIB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.715, 79.288, 87.295 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.240 - 1.360 |
R-factor | 0.1605 |
Rwork | 0.159 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.240 | 1.405 |
High resolution limit [Å] | 1.357 | 1.357 |
Rmerge | 0.052 | |
Rmeas | 0.055 | |
Rpim | 0.016 | |
Number of reflections | 60466 | 4228 |
<I/σ(I)> | 25.78 | 1.68 |
Completeness [%] | 95.2 | |
Redundancy | 11.9 | |
CC(1/2) | 0.999 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 277.15 | 0.1 M MES pH 6.2, 27% PEG 3350 |