7ZCM
Dark state structure of Sensory Rhodopsin II in complex with HtrII solved by room temperature serial synchrotron crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2019-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000342 |
Spacegroup name | P 21 2 2 |
Unit cell lengths | 52.870, 67.650, 114.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.650 - 2.850 |
R-factor | 0.3024 |
Rwork | 0.297 |
R-free | 0.34990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2s |
Data reduction software | careless |
Data scaling software | careless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.650 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 10066 | 998 |
<I/σ(I)> | 20.73 | |
Completeness [%] | 99.5 | |
Redundancy | 1.05 | |
CC(1/2) | 0.880 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 295 | PEG 400 38%, Calcium chloride 0.15 M, Glycine 0.1 M, pH 7.5 |