7ZCM

Dark state structure of Sensory Rhodopsin II in complex with HtrII solved by room temperature serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000342
Spacegroup name	P 21 2 2
Unit cell lengths	52.870, 67.650, 114.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.650 - 2.850
R-factor	0.3024
Rwork	0.297
R-free	0.34990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2s
Data reduction software	careless
Data scaling software	careless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.650	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	10066	998
<I/σ(I)>	20.73
Completeness [%]	99.5
Redundancy	1.05
CC(1/2)	0.880	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		295	PEG 400 38%, Calcium chloride 0.15 M, Glycine 0.1 M, pH 7.5