7ZC3
Crystal structure of human copper chaperone Atox1 bound to zinc ion by CxxC motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-11-12 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.2800 |
Spacegroup name | P 65 |
Unit cell lengths | 78.226, 78.226, 54.637 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.610 - 1.900 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.18590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qot |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Sir2014 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.040 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 23876 | 887 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 94.3 | 92 |
Redundancy | 2.3 | |
CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Drop:12.5mg/ml Atox1 + 1.62mM ZnSO4 in 25mM Sodium Phosphate buffer pH 7.0, 2mM DTT; Reservoir: 1.9M Li2SO4, 100mM MES pH 6.0, 2.5% glycerol; VAPOR DIFFUSION, SITTING DROP, temperature 293K. |