7ZBE

Dark state crystal structure of bovine rhodopsin in Lipidic Cubic Phase (SwissFEL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	294
Detector technology	PIXEL
Collection date	2020-07-24
Detector	PSI JUNGFRAU 1M
Wavelength(s)	1.38
Spacegroup name	P 2 21 21
Unit cell lengths	61.510, 91.010, 151.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.100 - 1.800
R-factor	0.215
Rwork	0.215
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u19
RMSD bond length	0.009
RMSD bond angle	0.912
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.100	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	79305	7852
<I/σ(I)>	7.62	0.95
Completeness [%]	100.0	100
Redundancy	830.6	556.7
CC(1/2)	0.993	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	9	294	36 % PEG 600, 100 mM Bicine pH 9.0