7ZAI
Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA and aIF1A.
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.6 |
| Number of Particle | 2000000 |
| Software | PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
