7ZA3
GPC3-Unc5D octamer structure and role in cell migration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.80000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 119.577, 119.577, 257.936 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.878 - 4.000 |
| Rwork | 0.355 |
| R-free | 0.38460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftt GPC3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.375 |
| Data reduction software | autoPROC |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.878 | 4.227 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.053 | |
| Rpim | 0.020 | |
| Number of reflections | 26038 | 1287 |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 73.8 | |
| Redundancy | 8.2 | |
| CC(1/2) | 1.000 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.8 | 291.15 | 0.1M Tris (pH 7.8) 5% w/v gamma-PGA, 20% w/vPEG 3350 |
