7Z8P
Crystal structure of YTHDF2 with compound ZA_166
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 277.15 |
| Detector technology | PIXEL |
| Collection date | 2022-01-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.080, 80.080, 114.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.100 - 1.970 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rdn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 58421 | 9377 |
| <I/σ(I)> | 16.01 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.36 | |
| CC(1/2) | 0.999 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2.05 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2 |
