7Z73
Crystal structure of p63 tetramerization domain in complex with darpin 8F1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-22 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 |
Unit cell lengths | 42.563, 50.761, 65.858 |
Unit cell angles | 80.93, 79.92, 68.75 |
Refinement procedure
Resolution | 47.060 - 2.270 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zuv 4a9z |
RMSD bond length | 0.011 |
RMSD bond angle | 1.244 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.060 | 47.060 | 2.350 |
High resolution limit [Å] | 2.270 | 8.790 | 2.270 |
Rmerge | 0.065 | 0.032 | 0.542 |
Rmeas | 0.076 | 0.038 | 0.628 |
Rpim | 0.038 | 0.019 | 0.318 |
Total number of observations | 82692 | 1511 | 8112 |
Number of reflections | 21523 | 397 | 2091 |
<I/σ(I)> | 11.3 | 30 | 2.2 |
Completeness [%] | 92.6 | 98.8 | 91.5 |
Redundancy | 3.8 | 3.8 | 3.9 |
CC(1/2) | 0.997 | 0.995 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M bis-tris pH 5.5 |