7Z4M
Plasmodium falciparum pyruvate kinase complexed with Mg2+ and K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 89.200, 109.670, 134.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.900 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3khd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.745 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | |
| Rpim | 0.024 | 0.638 |
| Number of reflections | 51642 | 5122 |
| <I/σ(I)> | 15.09 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.999 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES, pH 6.5, 50 mM KCl, 10 mM MgCl2, 14-24% PEG 600 |
