7Z2N

Tubulin-18-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	105.016, 157.703, 180.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.820 - 2.170
R-factor	0.1908
Rwork	0.189
R-free	0.22370
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.820	2.249
High resolution limit [Å]	2.170	2.170
Rmerge	0.116
Rmeas	0.120
Rpim	0.032	0.579
Number of reflections	157766	15493
<I/σ(I)>	16.69
Completeness [%]	99.9
Redundancy	13.7
CC(1/2)	0.999	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine