7YZW
Llama nanobody VHH15 raised against the glycoprotein of Ebola virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 142.671, 142.671, 160.307 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.240 - 1.600 |
R-factor | 0.1911 |
Rwork | 0.190 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bnw |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (16-JUL-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 126134 | 18171 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 100.0 | |
Redundancy | 20.1 | |
CC(1/2) | 1.000 | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0,1 M CAPS pH10.5 0,2 M Lithium sulfate 1,2 M sodium phosphate monobasic 0,8 M potassium phosphate dibasic |