7BNW

Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae

Summary for 7BNW

• Entry DOI: 10.2210/pdb7bnw/pdb
• Descriptor: nanobody nb18 (2 entities in total)
• Functional Keywords: nanobody, type ix secretion system, immune system
• Biological source: Lama glama (Llama)
• Total number of polymer chains: 4
• Total formula weight: 57344.16

Authors

Trinh, N.T.,Cambillau, C.,Leone, P. (deposition date: 2021-01-22, release date: 2021-05-26, Last modification date: 2024-11-13)

Primary citation

Trinh, T.T.N.,Gaubert, A.,Melani, P.,Cambillau, C.,Roussel, A.,Leone, P.
Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae.
Acta Crystallogr.,Sect.F, 77:171-176, 2021

PubMed Abstract: GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement.

PubMed: 34100775
DOI: 10.1107/S2053230X21005185

Experimental method

X-RAY DIFFRACTION (2.59 Å)