7YYP
Structure of aIF5B from Pyrococcus abyssi complexed with GDP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.420, 70.720, 74.260 |
| Unit cell angles | 90.00, 110.06, 90.00 |
Refinement procedure
| Resolution | 49.660 - 2.900 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fg3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.157 | 1.196 |
| Number of reflections | 15383 | 2452 |
| <I/σ(I)> | 9.08 | 1.28 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 6.9 | 6.3 |
| CC(1/2) | 0.997 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG3350; 0.2 M sodium nitrate |
