7YW3

Crystal structure of tRNA 2'-phosphotransferase from Homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	53.908, 84.062, 101.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.380 - 2.500
R-factor	0.2542
Rwork	0.252
R-free	0.29490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e3a
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.380	25.380	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.117	0.048	0.659
Rmeas	0.127	0.054	0.714
Rpim	0.050	0.024	0.274
Total number of observations	53150	973	6164
Number of reflections	8284	196	917
<I/σ(I)>	11.8	30.3	3.3
Completeness [%]	99.9	94.5	100
Redundancy	6.4	5	6.7
CC(1/2)	0.998	0.998	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1M Tris pH 8.0 28 % PEG4000