7YVD

The crystal structure of the progerin C-terminal peptide and the Ig-like domain of lamin A/C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97926
Spacegroup name	P 64 2 2
Unit cell lengths	91.974, 91.974, 204.562
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.390 - 2.100
R-factor	0.1659
Rwork	0.164
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7crg
RMSD bond angle	4.885
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.063	0.032	0.213
Rmeas	0.064	0.033	0.218
Rpim	0.011	0.005	0.044
Number of reflections	56656	1749	1487
<I/σ(I)>	16.9
Completeness [%]	99.7	98.8	99.7
Redundancy	28.7	35.1	22.5
CC(1/2)		1.000	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	287	0.1 M BIS-TRIS pH 6.5, 25% (v/v) Polyethylene glycol 300