7YVA

Crystal structure of Candida albicans Fructose-1,6-bisphosphate aldolase complexed with lipoic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	159.181, 56.622, 81.839
Unit cell angles	90.00, 96.33, 90.00

Refinement procedure

Resolution	45.370 - 2.930
R-factor	0.209
Rwork	0.204
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lnk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	3.040
High resolution limit [Å]	2.930	2.930
Number of reflections	15544	1493
<I/σ(I)>	9.38
Completeness [%]	98.5
Redundancy	3.3
CC(1/2)	0.998	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	22.5% (w/v) PEG 3350 100 mM Hepes pH =7.6