7YSF

Crystal structure of ZNF524 ZF1-4 in complex with telomeric DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	43.807, 52.194, 97.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.587 - 2.400
R-factor	0.2201
Rwork	0.218
R-free	0.25330
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.061	0.036	0.377
Rmeas	0.067	0.039	0.423
Rpim	0.026	0.016	0.189
Total number of observations	61403
Number of reflections	9248	533	419
<I/σ(I)>	11.7
Completeness [%]	99.4	99.8	89.5
Redundancy	6.6	5.8	4.7
CC(1/2)		0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Imidazole malate, pH 5.5, 15% (w/ v) polyethylene glycol 600