7YRU
ALK2 antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2015-05-26 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.048, 37.299, 118.638 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.600 |
| R-factor | 0.2468 |
| Rwork | 0.245 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 | 0.384 |
| Rmeas | 0.100 | 0.469 |
| Rpim | 0.057 | 0.265 |
| Number of reflections | 16127 | 1987 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 98.3 | 99.7 |
| Redundancy | 2.8 | 2.9 |
| CC(1/2) | 0.994 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 2% Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 20% PEG 3350 |
