7YQO

Xcc Nicotinamide Phosphoribosyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 2
Unit cell lengths	91.610, 151.480, 70.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.810 - 1.790
R-factor	0.1788
Rwork	0.178
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e68
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.610	8.010
High resolution limit [Å]	1.790	1.790
Rmerge	0.109
Number of reflections	93464	6845
<I/σ(I)>	16.8
Completeness [%]	99.9
Redundancy	13.085
CC(1/2)	0.999	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Citric acid, BIS-TRIS propane, Polyethylene glycol 3350), GSH (L-Glutathione reduced), 0.01 M GSSG