7YPC

Notothenia coriiceps TRAF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	111.859, 112.208, 112.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.770 - 2.660
R-factor	0.2079
Rwork	0.207
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k8u
RMSD bond length	0.010
RMSD bond angle	1.144
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.773	2.755
High resolution limit [Å]	2.660	2.660
Number of reflections	40583	3392
<I/σ(I)>	17.2
Completeness [%]	98.4
Redundancy	13.2
CC(1/2)	0.999	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M HEPES pH 7.5, 10% w/v PEG-8000, 8% v/v Ethylene glycol